RSA 키 (2048)

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-----BEGIN PUBLIC KEY----- MIIBIjANBgkqhkiG9w0BAQEFAAOCAQ8AMIIBCgKCAQEAt8hGha2sHvPZcgBcdt/p SUl4tyiSnWotBL1WuBXXR69nsT9lSlxvi4qQyUPQ3JVddfzsIH5iRxUPA9Lkne5z hMb5iz72KciT1a5RJNWGpryAqlxe+roQ1vAyKCjhGQM1My9tn4V3TyqEjuHu4oqF LFUArp7WTgNVjTot3QJ20IpeHXFbEGGO0AQZnWUyjvEiAPlB3DFUP5S5tns1eGMW NQ0ytsVg9IVt3IHBRftww8w853CSGGdasICCZr99Zc4CHj40L4+TNK2Gc9cA7P+j AE2J+/7ceQnuUv+fGqoEWK2EGdC3lL3o3Pp7yIrAiL7i2rX44+ncGhRNGtv2onS7 MQIDAQAB -----END PUBLIC KEY-----

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D: 21 April14. fifteen.16.17. 18. 19.20. 21. 22.References one. Choi J…

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작성자 Meredith
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D: 21 April14. 15.sixteen.17. 18. 19.twenty. 21. 22.References one. Choi JH, Lee SY. Secretory and extracellular creation of recombinant proteins applying Escherichia coli. Appl Microbiol Biotechnol. 2004;64:625?5. 2. Nijland R, Kuipers OP. Optimization of protein secretion by Bacillus subtilis. Latest Pat Biotechnol. 2008;2(2):1?. 3. Westers L, Westers H, Quax WJ. Bacillus subtilis as cell manufacturing facility for pharmaceutical proteins: a biotechnological method of optimize the host organism. Biochim Biophys Acta. 2004;1694:299?10. 4. van Dijl JM, Hecker M. Bacillus subtilis: from soil bacterium to super-secreting cell manufacturing facility. Microb Mobile Simple fact. 2013;twelve:3. 5. Phan TT, Nguyen High definition, Schumann W. Enhancement of the powerful intracellular expression system for Bacillus subtilis by optimizing promoter features. J Biotechnol. 2012;157:167?2. six. Kunst F, Ogasawara N, Moszer I, Albertini AM, Alloni GO, Azevedo V, Bertero MG, Bessieres P, Bolotin A, Borchert S, Borriss R. The complete genome sequence in the gram-positive bacterium Bacillus subtilis. Character. 1997;390(6657):249?6. 7. Barbe V, Cruveiller S, Acetaminophen Kunst F, Lenoble P, Meurice G, Sekowska A, Vallenet D, Wang T, Moszer I, Medigue C, Danchin A. From the consortium sequence to the unified sequence: the Bacillus subtilis 168 reference genome a decade afterwards. Microbiology. 2009;one hundred fifty five:1758?five.23. 24. 25. 26.27.28.Kobayashi K, Ehrlich SD, Albertini A, Amati G, Andersen KK, Arnaud M, Asai K, Ashikaga S, Aymerich S, Bessieres P, et al. Important Bacillus subtilis genes. Proc Natl Acad Sci. 2003;one hundred:4678?three. Tjalsma H, Antelmann H, Jongbloed JD, Braun PG, Darmon E, Dorenbos R, Dubois JY, Westers H, Zanen G, Quax WJ, et al. Proteomics of protein secretion by Bacillus subtilis: separating the "secrets" on the secretome. Microbiol Mol Biol Rev. 2004;68:207?3. Antelmann H, Bron S, Hecker M. Proteomic survey through secretome of Bacillus subtilis. In: Humphery I, Hecker M, editors. Microbial proteomics: useful biology of complete organisms. Hoboken: Wiley; 2006. Bendtsen JD, Nielsen H, Widdick D, Palmer T, Brunak S. Prediction of twinarginine signal peptides. BMC Bioinformatics. 2005;6:167. Lin FL, Rong XZ, Fen LW, Bing SJ, Ping L, Xia HC. Protein secretion pathways in Bacillus subtilis: implication for optimization of heterologous protein secretion. Biotechnol Adv. 2007;25:one?2. Caspers M, Brockmeier U, Degering C, Eggert T, Freudl R. Advancement of Sec-dependent secretion of a heterologous product protein in Bacillus subtilis by saturation mutagenesis on the N-domain in the AmyE signal peptide. Appl Microbiol Biotechnol. 2010;86:1877?5. Heng C, Chen Z, Du L, Lu F. Expression and secretion of the acid-stable alpha-amylase gene in Bacillus subtilis by SacB promoter and sign peptide. Biotechnol Lett. 2005;27:1731?. Chen J, Gai Y, Fu G, Zhou W, Zhang D, Wen J. Enhanced extracellular output of alpha-amylase in Bacillus subtilis by optimization of regulatory aspects and over-expression of PrsA lipoprotein. Biotechnol Lett. 2015;37:899?06. Liu R, Zuo Z, Xu Y, Tune C, Jiang H, Qiao C, Xu P, Zhou Q, Yang C. Twin-arginine sign peptide of Bacillus subtilis YwbN can direct Tatdependent secretion of methyl parathion hydrolase. J Agric Foodstuff Chem. 2014;sixty two:2913?. Wang G, Chen H, Zhang H, Tune Y, Chen W. The secretion of the intrinsically disordered protein with unique secretion indicators in Bacillus subtilis. Curr Microbiol. 2013;sixty six:566?2. Wang G, Xia Y, Tune PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/7500280 X, Ai L. Common non-classically secreted bacterial proteins with experimental proof. Cu.

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